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MOLECULAR BIOlogy - SEMESTER 2 LECTURE NOTES

Module Descriptor
​
Sample Exam Questions
File Size: 135 kb
File Type: pdf
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Topic 1
An Introduction to
​Molecular Biology


Handout 1
File Size: 10047 kb
File Type: pdf
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Larger images (1)
File Size: 7788 kb
File Type: pdf
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Topic 2
​Replication

Handout 2
File Size: 7152 kb
File Type: pdf
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Larger images (2)
File Size: 5248 kb
File Type: pdf
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Watch the interactive Replication animation we covered in class HERE! (needs 'flash' installed work - use a computer vs tablet/phone)...& watch the topisomerase video clip HERE!

DNA Replication; Revision Material

DNA replication simulation (yourgenome.org)


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Exercise 1
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Exercise 2
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Exercise 3
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Exercise 4
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Exercise 5

DNA Replication revision quiz on Socrative.  
You can access the quiz and take it as often as you wish HERE​.
The room name is REPLICATE
Best of luck

 
Topic 3
Transcription

Handout 3
File Size: 8004 kb
File Type: pdf
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Larger images (3)
File Size: 6402 kb
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From class; transcription animation HERE ; 3′ poly A tail animation HERE! ; Splicing HERE

Transcription and mRNA Processing; Revision Material



 
Topic 4
​
Methods review and recombinant gene technology
Handout 4
File Size: 9692 kb
File Type: pdf
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Methods - qPCR.pdf
File Size: 3414 kb
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Larger images (4)
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Larger Images - qPCR.pdf
File Size: 5397 kb
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Links to the animations can be accessed here also:  Agarose gel electrophoresis, restriction enzymes animation 1 and 2, PCR 1, 2 and 3, RT-PCR,  Southern Blotting,

Revision video for molecular methods

Complementing our methods handout, the pre-practical video playlist on YouTube represents a nice revision too.

Just click the links in this box to go to the relevant video!  
Revision slides on calculations
File Size: 240 kb
File Type: pdf
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  • Using autopipettes in the Laboratory
  • Making a buffer
  • DNA Extractions
  • UV Spectrophotometry and DNA
  • Agarose Gel Electrophoresis
  • Polymerase Chain Reaction (PCR)
  • Restriction Enzymes

PCR Review Quiz:
Click here to access Socrative and enter the room number:   PCRREVIEW
(23 question quiz covering the PCR; you can take it more than once).


 
Topic 5
Translation

Handout 5
File Size: 6193 kb
File Type: pdf
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Larger images (5)
File Size: 4013 kb
File Type: pdf
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Translation overview animations can be seen here & here

Translation Review Quiz:
Click here to access Socrative and enter the room number:   TRANSLATE
(23 question quiz covering the 3 mini-lecture videos above; you can take it more than once).

Topic 6
Some recent Developments in Molecular Biology


Gene Editing with CRISPR technology


How CRISPR lets us edit our DNA Jennifer Doudna (16 mins)
What you need to know about CRISPR Ellen Jorgensen (10 mins)
How CRISPR edits? 
​TedEd (5 mins)

Guardian CRISPR article worth reflecting on:  "Gene editing like CRISPR is too important to be left to scientists alone"

Nature news article (March 2020):  CRISPR treatment inserted directly into the body for first time

CRISPR and Coronavirus: A Cell paper (April 2020); just look at the short abstract on their page 2 (3rd page!)
​Development of CRISPR as an antiviral strategy to combat SARSCoV-2 and influenza


Other Resources:
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  • Home
  • About
  • Modules ↓
    • Second Year Students >
      • Biochemistry (Semester 1) >
        • Lecture notes sem 1
        • Practical resources
      • Molecular Biology (Semester 2) >
        • Lecture notes sem 2
        • Practical resources
    • Y3 Project
    • Biopharma Therapeutics >
      • Biopharma therapeutics notes
    • Upstream Processing >
      • Upstream notes
      • Video project
  • Facebook
  • Careers
    • Recruitment Agents & Job Sites
    • CV Tips
    • Important interview questions
    • Sample Y4 graduate jobs
  • Student Resources
  • Contact