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Topic 1 An Introduction to Molecular Biology |
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Topic 2 Replication |
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Topic 3 Transcription |
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Revision videos for Transcription
Video 1: YouTube link here. Timestamp hyperlinks: 00:17 Transcription 'Bubble' 01:49 Flow of information from gene to protein; what is a gene?, a promoter? etc. 06:58 The role of the template and coding strands 08:39 3 stages; initiation, elongation and termination 13:45 Summary |
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Topic 4 Methods review and recombinant gene technology |
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Links to the animations can be accessed here also: Agarose gel electrophoresis, restriction enzymes animation 1 and 2, PCR 1, 2 and 3, RT-PCR, Southern Blotting, | ||||||||
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Revision video for molecular methods
Complementing our methods handout, the pre-practical video playlist on YouTube represents a nice revision too. Just click the links in this box to go to the relevant video! |
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Plan for week commencing 23rd March 2020
Recently, we were discussing standard PCR and also qPCR (quantitative, real time, PCR).
You can download the specific slides/images in the two methods handouts linked above.
Recently, we were discussing standard PCR and also qPCR (quantitative, real time, PCR).
You can download the specific slides/images in the two methods handouts linked above.
1. The PCR topic: Please watch my three mini-lecture videos below covering various aspects as indicated below.
2. Complete the Quiz below in the yellow box below the PCR videos (feedback provided to assist your learning).
3. Ensure you have performed the PCR pre-practical quiz (see 13th March e mail from me). Important to do this.
4. Regarding your last lab, complete this virtual lab on PCR and also this one on gel electrophoresis by Friday 3rd April. Note that with the agarose gel, we stain with SYBR safe during the process, while in this example they stain the DNA at the end with a different chemical. As a 'virtual lab manual exercise', please submit one 250 word (max) summary of these virtual sessions to Moodle by April 3rd.
5. Reminder: CA exam takes place at 1pm online on Friday 27th March (see recent e mail sent last Friday).
2. Complete the Quiz below in the yellow box below the PCR videos (feedback provided to assist your learning).
3. Ensure you have performed the PCR pre-practical quiz (see 13th March e mail from me). Important to do this.
4. Regarding your last lab, complete this virtual lab on PCR and also this one on gel electrophoresis by Friday 3rd April. Note that with the agarose gel, we stain with SYBR safe during the process, while in this example they stain the DNA at the end with a different chemical. As a 'virtual lab manual exercise', please submit one 250 word (max) summary of these virtual sessions to Moodle by April 3rd.
5. Reminder: CA exam takes place at 1pm online on Friday 27th March (see recent e mail sent last Friday).
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1. PCR overview & controls
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2. Reverse Transcription PCR
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3. qPCR (real time PCR)
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00:00 Introduction
PCR pre-practical video to watch 01:21 The PCR process 07:18 Controls in PCR ; Exercise 17:43 Summary & applications |
00:00 Introduction
00:23 Comparison of standard PCR and qPCR 01:22 What a qPCR machine measures (SYBR green in qPCR) 05:12 Comparison of qPCR approaches 08:16 Points to become aware of 09:11 How qPCR results are interpreted (Ct value determination) 16:15 The melting curve analysis 20:09 Review questions |
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PCR Review Quiz:
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Click here to access Socrative and enter the room number: PCRREVIEW
(23 question quiz covering the 3 mini-lecture videos above; you can take it more than once). |
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Topic 5 Translation |
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Plan for week commencing 30th Mar 2020 (week 11)
& week commencing 20th April 2020 (week 12 after Easter break)
& week commencing 20th April 2020 (week 12 after Easter break)
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NDSU Virtual Cell Translation Video
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1. Translation (Part 1)
00:28 Points we are going to cover
01:56 Where is translation in the flow of information 03:49 Some key points on Translation 07:13 Definition of Translation 09:16 Starting translation/AUG/reading frames 09:56 Our key diagram 19:10 An adaptor molecule that speaks two languages 20:57 How a codon is linked to a specific amino acid 22:09 Impact of changes to the code/degeneracy of the genetic code 27.39 An amino acid can't recognise a codon 28:19 The anticodon-codon relationship 29:18 Exercise for you |
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Translation Review Quiz:
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Click here to access Socrative and enter the room number: TRANSLATE
(23 question quiz covering the 3 mini-lecture videos above; you can take it more than once). |
Topic 6
Some recent Developments in Molecular Biology
Some recent Developments in Molecular Biology
Gene Editing with CRISPR technology
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How CRISPR lets us edit our DNA Jennifer Doudna (16 mins)
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What you need to know about CRISPR Ellen Jorgensen (10 mins)
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How CRISPR edits?
TedEd (5 mins) |
Guardian CRISPR article worth reflecting on: "Gene editing like CRISPR is too important to be left to scientists alone"
Nature news article (March 2020): CRISPR treatment inserted directly into the body for first time
CRISPR and Coronavirus: A Cell paper (April 2020); just look at the short abstract on their page 2 (3rd page!)
Development of CRISPR as an antiviral strategy to combat SARSCoV-2 and influenza
Development of CRISPR as an antiviral strategy to combat SARSCoV-2 and influenza
Some recent COVID-19 science articlesWhile a rapidly evolving area, here are a few recent articles on the science of COVID-19 that may be of interest to read up on.
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Please ensure you obtain your information from trusted sources. A lot of "opinions" out there, so always consider the quality of your sources. |
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In this video, UC Berkeley professor and IGI Investigator Britt Glaunsinger explains the evolution, genetics, and virulence of coronaviruses. Watch first 20 mins.
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Principle of probe based qPCR – approach being used in COVID testing. Requires primers and a ‘probe’ (3 mins).
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Some recent articles that may be of interest
....click links below to read
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A podcast worth considering...Short, snappy and up to date episodes…well worth a listen – you can even submit questions! |
Other Resources:
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